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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been observed in many tumors, which results in chromosome instability, and so may possibly contribute to tumorigenesis. This protein was found to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a critical job in advertising mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have already been described. Two further splice variants have been claimed but comprehensive nucleotide sequence information and facts isn't yet readily available.
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Blocking peptides are peptides that bind precisely to the target antibody and block antibody binding. These peptide normally contains the epitope acknowledged because of the antibody.
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This tutorial outlines more probable optimization of cellular and immunoassay parameters to guarantee the best possible effects are...
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This information outlines even more doable optimization of cellular and immunoassay parameters to be certain the best possible success are...
Antibodies certain to the blocking peptide no longer bind to the epitope to the focus on protein. By evaluating the staining from your blocked antibody compared to the antibody alone, one can see which staining is specific.
Goal Info Cyclin E belongs towards the extremely conserved cyclin relatives, whose customers are characterized by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Various cyclins show unique expression and degradation designs which contribute towards the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for mobile cycle G1/S transition. This protein accumulates with the G1-S period boundary and is particularly degraded as cells development through S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...
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